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Confirmation of differential mRNA expression of Klk2 and Pttg1 using quantitative RT-PCR. cDNAs from WT, Grk1−/−, Nrl−/−, and Nrl−/−Grk1−/− 1-month-old retinas were used for quantitative expression analysis, and β-actin was a reference standard. Statistically significant upregulation of (A) Pttg1 and (B) Klk2 was observed in both Grk1−/− compared with WT and Nrl−/−Grk1−/− compared with Nrl−/−. *P < 0.05; **P < 0.01.
