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MS/MS spectra of ammoniated WE peaks: (a) a standard (behenyl oleate 18:1/22:0, ∼30 μg/mL) and (b) a WE mixture in meibum (total lipid concentration ∼16 μg/mL). The solutions were prepared in chloroform/methanol mixture (2:1, v/v) with 1 mM ammonium acetate additive. The WE mixtures in meibum are composed of species with the composition of fatty acid/fatty alcohol moieties being 18:1/22:0 and 16:1/24:0.
