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Effect of activation of Epac on MAPK activity. Lacrimal gland acini were incubated with the 8-pCPT-2Me-cAMP, a specific activator of Epac, (10−9–10−3 M) for 30 minutes. Western blot analysis was performed using an antibody against phosphorylated p42/p44 MAPK. Data are mean ± SEM of six independent experiments (A). *Significant difference from basal. Representative blot (B).
