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. 2010 Dec;51(12):6566–6574. doi: 10.1167/iovs.10-6161

Figure 2.

Figure 2.

Effect of cyclophosphamide on ACAID and corneal allograft survival. Cyclophosphamide treatments were performed 1 day before and once weekly after AC injection or corneal transplantation. (A) ACAID was induced on day 0 with C57BL/6 splenic nonadherent cells. A SC injection of C57BL/6 splenocytes was given on day +7. DTH challenge with mitomycin C-treated C57BL/6 cells was administered on day +14. Negative control animals received an ear challenge only. Positive and DTH control animals were immunized SC and received an ear challenge but were not injected in the AC. P = 0.001 for cyclophosphamide-treated versus untreated group in which ACAID was induced (n = 5). The experiment was performed twice with similar results. (B) C57BL/6 corneal allograft survival in BALB/c mice treated with cyclophosphamide. C57BL/6 corneal allografts underwent rejection in 50% of untreated recipients (n = 10) and had an MST of 52 days. BALB/c hosts treated with cyclophosphamide rejected 80% of grafts with an MST of 28 days (n = 10). P < 0.05 between cyclophosphamide-treated and untreated allograft recipients. No rejection was observed in the syngeneic recipient treated with cyclophosphamide.