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. 2010 Dec;51(12):6582–6592. doi: 10.1167/iovs.10-5626

Figure 3.

Figure 3.

Expression of Aqp0a and Aqp0b in larval and adult zebrafish lens. (A) Western blot analyses were performed on membrane proteins from zebrafish 3-day-old, 1-year-old, 2-year-old, and 3-year-old lenses using antibodies directed against Aqp0a and Aqp0b, respectively. Both antibodies recognized a band at 26 kDa demonstrating the presence of Aqp0a and Aqp0b in the larval and adult zebrafish lens. Additional bands (arrowheads) are detected at lower molecular weight that could indicate the cleavage of Aqp0a and Aqp0b during aging. (B) Western blot analysis was performed on membrane proteins from Xenopus oocytes uninjected or expressing bovine AQP0, Aqp0a, or Aqp0b. The antibody directed against Aqp0a recognized a band at 26 kDa in the membranes of oocytes expressing Aqp0a. Similarly, the antibody directed against Aqp0b recognized a band at 26 kDa in the membranes from oocytes expressing Aqp0b. These Western blots demonstrate the specificity of the anti-Aqp0a and anti-Aqp0b antibodies. The anti-Aqp0b antibody also recognized a band at 40 kDa in the oocytes expressing Aqp0b, presumably a posttranslational modification of Aqp0b.