Figure 4.

Aqp0a and Aqp0b morphants developed cataract. (A) Microscopic observation with Hoffman optics of Aqp0a, Aqp0b, and double morphants revealed marked opacity and roughness in the lens core at 3 dpf. In contrast, the control lenses were smooth looking. (B) Aqp0a and Aqp0b expression in morphants eyes. Western blot analysis was performed on membrane proteins from eyes from 3 dpf control embryos and Aqp0a morphants, Aqp0b morphants, and double morphants using antibodies directed against Aqp0a and Aqp0b, respectively.