Figure 6.

Aqp0a and Aqp0b water permeability in yeast spheroplasts. Water permeability was tested for yeast spheroplasts expressing Aqp0a or Aqp0b. Spheroplasts containing a blank vector were used as a control. Control spheroplasts and spheroplasts expressing Aqp0b displayed increased absorption at 600 nm (significant shrinking) over the course of the 20-second experiment, resulting in a positive ΔOD₆₀₀. Spheroplasts expressing Aqp0a shrank nearly instantaneously with the hyperosmotic shock and were, therefore, shrunk before the first time point of the 20-second experiment, resulting in a lower magnitude ΔOD₆₀₀. Water permeability and ΔOD₆₀₀ were inversely related. Aqp0b water permeability was not statistically different from that of the control (P > 0.1). *P < 0.02 using the Student's t-test.