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. Author manuscript; available in PMC: 2011 Mar 11.
Published in final edited form as: Lab Invest. 2010 May 10;90(7):1078–1090. doi: 10.1038/labinvest.2010.90

Figure 2.

Figure 2

Hypoxia inhibits angiogenesis and TSP-1 expression in normal prostate epithelial cells while angiogenesis is induced in PCa cells. Normal prostate epithelial cells (PrEC) and androgen sensitive LNCaP and androgen-insensitive PC-3 PCa cells grown under standard conditions (normoxia, N) or hypoxia-treated conditions [50 μM CoCl2 (C) or in a hypoxia chamber (H), 0.5% O2] and serum-free conditioned media were collected. (a) Angiogenic activity of PC-3 and LNCaP conditioned media (20 μg/ml) was assessed using a microvascular endothelial cell migration assay. Data are presented as mean ± SE of the percent maximum migration toward the positive control. (b) VEGF levels were assayed by ELISA, and data are presented as mean ± SD. (c) Angiogenic activity (d) and VEGF levels were evaluated in PrEC as above. (e) TSP-1 expression was evaluated by Western blot. The lower panel is an identically loaded gel to show equal protein loading between lanes. Protein levels relative to cells grown in normoxia were determined by densitometry. Protein size standard is indicated. *Significantly different compared to cells grown under normoxic conditions, P<0.05.