Fig. 1. Bbp1p interacts with Spc29p. (A) BBP1 is a high gene dosage suppressor of the temperature-dependent growth defect of spc29(ts) cells. Cells of spc29-2 and spc29-3 with the indicated plasmids were incubated for 3 days at 35°C. (B) Two-hybrid interactions between Spc29p and Bbp1p. Blue colony colour indicates interaction. The control cells carry e.g. the pMM5 derived LexA-BBP1 plasmid combined with pMM6 without insert. (C) Binding of Spc29p to Bbp1p. ³⁵S-labelled Spc29p was incubated with purified GST (lane 1) or GST–Bbp1p (lane 2) bound to glutathione–Sepharose beads. The bound proteins were eluted and analysed by immunoblotting with anti-Bbp1p antibodies or by autoradiography ([³⁵S]Spc29p).