Fig. 5. Many bbp1-1 cells fail to assemble a nuclear inner plaque. α-factor-synchronized bbp1-1 cells containing (A) SPC110-GFP or (B) SPC42-GFP were shifted to the restrictive temperature for 1.5 h. Cells were fixed with paraformaldehyde and DNA was stained with DAPI. Approximately half of large budded bbp1-1 SPC42-GFP cells (54%) carried two SPB signals. Only 16% of bbp1-1 SPC110-GFP cells with two SPB signals were detected. Bar, 5 μm.