Fig. 7. Bbp1p binds directly to Mps2p. (A–C) GST–Bbp1p (lanes 1 and 3), GST–C-Bbp1p202–385 (lanes 4 and 5), GST–N-Bbp1p1–237 (lanes 6 and 7) and GST (lanes 8 and 9) bound to glutathione–Sepharose were incubated with (lanes 3, 5, 7 and 9) or without (lanes 1, 4, 6 and 8) purified His6-N-Mps2p1–307 (lane 2). The GST–Bbp1p derivatives and associated proteins were eluted with sample buffer. The eluate was analysed by Coomassie Blue staining (A) and by immunoblotting with anti-GST (B) or anti-His tag (C) antibodies. (A) Protein bands (lanes 2, 3, 5 and 7) corresponding in size to His6-N-Mps2p1–307 are marked by an asterisk.