Fig. 1. Normal androgen action and internal genitalia of TLS–/– mice. (A) TLS, EWS and TAFII68 Western blots of whole-cell extracts from mouse embryonic fibroblasts with the TLS genotypes indicated. (B) Photomicrograph of the internal genitalia of adult (9–week-old) wild-type and TLS–/– male sibling mice. The bar corresponds to 5 mm. (C) Northern blot of kidney mRNA from female and castrated male mice injected with testosterone (4 μg/g/day) and hybridized with the androgen-responsive SA gene (upper panel) and β–tubulin (lower panel). Note the normal androgen responses of the mutant mice.