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Effect of escalating concentrations of combretastatin A-4 and colchicine on the inhibition of the bisthioalkylation of Cys-239 and Cys-354 of β-tubulin by N,N'-ethylene-bis(iodoacetamide) on MDA-MB-231 cell lines. The experiments for panels A and B were performed using SDS 65% while SDS 98.5% was used for panel C.
