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Absolute quantification of miRNAs in L. albus phloem exudate and pod tissue. 0.5 μg of total RNA isolated from pod tissue and phloem exudate was reverse transcribed using miRNA-specific stem-loop primers followed by real-time PCR analysis performed on a LightCycler480 (Roche Diagnostics) using SYBR® green as the fluorescent dye. Data are the mean ± standard deviation of three biological replicates with two technical replicates each.
