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. 2011 Mar 11;6(3):e17852. doi: 10.1371/journal.pone.0017852

Figure 1. Reverse transcription (RT)-PCR of bicP and bpss1522 in wild-type and bopA mutant B. pseudomallei.

Figure 1

RNA extracted from the wild-type K96243 or bopA deletion mutant strain was reverse-transcribed to obtain cDNA. Each cDNA preparation was used as a template for RT-PCR. (A) Schematic diagram showing the position of primers used for RT-PCR. (B) Electrophoretic separation of RT-PCR products amplified with primers for bicP (top panel) and bpss1522 (lower panel). These products were generated from reactions: (2) bopA mutant cDNA; (3) bopA mutant cDNA, no RT control; (4) wild-type cDNA, (5) wild-type cDNA, no RT control; (6) wild-type genomic DNA control; and (7) No DNA control. DNA size markers (bp) are shown in lane 1.