Figure 3.

Determination of R_s of BsSMC by gel filtration. The column was calibrated by running standard proteins BSA, catalase, and thyroglobulin over the column, then BsSMC. BsSMC eluted in fraction 14.2, which corresponds to an R_s of 10 nm on the extrapolated curve. In repeated experiments, the average R_s was 10.3 nm [19].