EtOH exposure impairs GLUT1 expression in brain tissues: immunohistochemistry of GLUT1 (green) in brain tissue sections merged with vWF (red) in control (a, b), EtOH (c, d), and EtOH + ALC (e, f).g Western blot analysis of 45-kDa isoform GLUT1 protein in brain tissue protein extract. h Results are expressed as the ratio of GLUT1 to α-actin bands and presented as mean values (± SD; n=3). **p<0.01 statistically significant compared with control. Scale bar indicated 20 μmin a–f