Fig. 3. Partial proteolysis of Vph1p in wild-type and vma22Δ cells. Microsomes were prepared from wild-type (SNY28) and vma22Δ (KHY38) cells and treated for various times with subtilisin (0.8 μg/ml). Precipitated proteins were resolved by SDS–PAGE and immunoblots probed with either anti-Vph1p (A) or anti-Kar2p (B) antibodies. The single asterisk (^*) indicates a non-specific band present in vma22Δ immunoblots and ^** indicates the same non-specific band present in wild-type immunoblots when overexposed.