Fig. 4. Degradation of unassembled Vph1p is an energy-requiring process. vma22Δ (KHY125/pMM322) cells were radiolabeled, chased in either the absence or presence of 20 mM sodium azide and aliquots harvested at various times. After immunoprecipitation with anti-Vph1p antibodies, the Vph1p-depleted extracts were then used to immunoprecipitate CPY^*. After separation by SDS–PAGE, the amount of radioactivity in each sample was quantified using a Storm phosphorimager; the 0 min chase time quantity was designated as 100% and the percentage of protein remaining was plotted against time. To determine half-lives, first order decay curves were generated using average values from at least three independent experiments.