Fig. 8. Cytosolic but not lumenal molecular chaperones are involved in the turnover of unassembled Vph1p. KHY190 (vma22Δ kar2-1 prc1-1) and KHY181 (vma22Δ KAR2 prc1-1) cells were radiolabeled at 24°C and chased at 37°C. Aliquots were taken at specific time points, the cells lysed and Vph1p immunoprecipitated from solubilized extracts (A). Vph1p-depleted extracts were then used to immunoprecipitate CPY^* (B). ACY76 (vma22Δ ssa1-ts ssa2 ssa3 ssa4) cells carrying either pRS313 or pAC427 (SSA1) were radiolabeled at 24°C and chased at either 24°C (C) or 37°C (D). Vph1p was then immunoprecipitated as described.