Fig. 4. Inhibition of isoflurane-increased co-immunoprecipitation of PKCα with EAAT3 by synthetic peptides.
C6 cells were incubated with or without 2% isoflurane in the presence or absence of 10 μM peptides for 5 min at 37°C and the total lysates were immunoprecipitated with an anti-EAAT3 antibody. A representative Western blot of the precipitates is shown in the top panel and the graphic presentation of the PKCα protein abundance quantified by integrating the volume of autoradiograms from four separate experiments is shown in the bottom panel. * P < 0.05 compared with the control. ˆ P < 0.05 compared with isoflurane alone.