Fig. 1.

NB54- and NB84-mediated suppression of premature stop mutations. Western blot analysis of protein samples prepared from aminoglycoside-treated and untreated transiently transfected HeLa cells, using a monoclonal anti-FLAG antibody or a specific antibody detecting the C terminus of MeCP2. Thirty microgram of protein extract was loaded. Arrows denote the full-length FLAG-MeCP2 protein as well as the truncated FLAG-MeCP2 isoforms. As a loading control, GAPDH was detected in all samples. The cells were treated for 24 h with 500 μg/ml of gentamicin (a), NB30 (a), NB54 (a–b), or NB84 (b). The effect of 24-h drug treatment was quantified by densitometric western blot analysis from at least three independent experiments (c)