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. Author manuscript; available in PMC: 2012 Feb 15.
Published in final edited form as: Circulation. 2011 Jan 31;123(6):584–593. doi: 10.1161/CIRCULATIONAHA.110.971853

Figure 8. EPA and DHA increase NOx production, phospho-eNOS and eNOS expression in cardiac fibroblasts.

Figure 8

(A) The levels of nitrite/nitrate (NOx) in the cell were determined using a Total Nitrate/Nitrite Fluorometric method in cultured cardiac fibroblasts treated for 24 hr with EPA (10 μM), DHA (10 μM), 8-bromo-cGMP (0.1 mM), or 8-bromo-cGMP (1 mM) (n=4–14). (B) Phospho-eNOS and eNOS detected by western blot in cultured cardiac fibroblasts treated for 24 hr with EPA or DHA (10 μM). (C, D) Quantification of phospho-eNOS or eNOS relative to GAPDH (n=7–9). Data are presented as mean ± SEM. Data were analyzed by one-way ANOVA with Tukey’s post-hoc test.