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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Biochemistry. 2010 Jun 1;49(21):4516–4523. doi: 10.1021/bi1003684

Fig 1. Effect of N-ethylmaleimide on the association state of spectrin in the red cell membrane and in solution.

Fig 1

A. Native polyacrylamide gel showing separation of spectrin dimer (D) and tetramer (T), after extraction from cells treated with the indicated concentrations of NEM. B. Proportion of spectrin converted to dimer in situ, determined by densitometry of the gels. C. Polyacrylamide gel and D. densitometric evaluation of equilibrium mixture of spectrin dimer and tetramer after treatment in solution with the indicated concentrations of NEM.