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. 2010 Nov-Dec;1(6):383–387. doi: 10.4161/gmic.1.6.13726

Figure 2.

Figure 2

Effect of lysozyme on release of soluble TLR-stimulants from Gram-negative bacteria. E. coli (109/ml), B. fragilis (109/ml), Pam3CSK4 (1 µg/ml) and LPS (1 µg/ml) were resuspended in PBS with or without 1 mg/ml lysozyme for 2 h at 37°C. Filtered supernatants were then diluted 1:100 in tissue culture medium and applied to RAW macrophages for the measurement of secreted TNFα (A) or HEK-293 cells transfected with TLR2 (B) or TLR4/MD2 (C) for the measurement of TLR-dependent NFκB signalling. *p < 0.05 (ANOVA with Tukey's test).