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Alkyne–SAM assessment in lysine methylation assays. A) SETDB1 was incubated with GST–H3tail and 1 or controls (no cofactor, trace material from analytical HPLC prep). Following the methyltransferase reaction, the product was treated with the azide-FLAG and the samples were analyzed by SDS-PAGE and immunoblotting by using the indicated antibodies. B) Methyltransferase reactions were performed as in panel A, but by using 1 in increasing amounts.
