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. 2010 Nov 17;12(2):330–334. doi: 10.1002/cbic.201000433

Scheme 1.

Scheme 1

A chemical biology method for labeling lysine methyltransferase substrates. A) Alkyne–SAM analogue 1. B) PKMT substrate labeling method: enzymatic transfer by a PKMT of a terminal alkyne from 1 to a protein substrate is followed by CuAAC to ligate an azido-FLAG epitope to the substrate protein.