Table 1.
Dioxygenases activity after induction by aromatic substrates (4-HB-4-hydroxybenzoic acid; 3,4-DHB-3,4-dihydroxybenzoic acid; HQ-hydroquinone; 2-NP-2-nitrophenol; 3-NP-3-nitrophenol; 4-NP-4-nitrophenol; the plus/minus values represent standard deviation)
| Enzyme | Catechol 1,2-dioxygenase, U/mg protein | Catechol 2,3-dioxygenase, U/mg protein | Protocatechuate 3,4 -dioxygenase U/mg protein | Protocatechuate 4,5 -dioxygenase U/mg protein | Hydroquinone 1,2 -dioxygenase, U/mg protein |
|---|---|---|---|---|---|
| Inductor | Substrate in reaction mixture | ||||
| Catechol | Catechol | 3,4-DHB | 3,4-DHB | HQ | |
| Phenol | 0.06 ± 0.02 | 4.92 ± 0.34 | 0.00 ± 0.00 | 0.00 ± 0.00 | ND |
| 4-HB | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.13 ± 0.05 | 0.00 ± 0.00 | ND |
| 3,4-DHB | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.46 ± 0.13 | 0.00 ± 0.00 | ND |
| Benzoate | 11.97 ± 0.34 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | ND |
| 2-NP | 0.29 ± 0.13 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.11 ± 0.00 | 0.00 ± 0.00 |
| 3-NP | 0.12 ± 0.01 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| 4-NP | 0.14 ± 0.08 | 0.01 ± 0.00 | 0.00 ± 0.00 | 0.73 ± 0.00 | 0.00 ± 0.00 |