Table 2.
Dioxygenases activity in cometabolic cultures (4-HB-4-hydroxybenzoic acid; 3,4-DHB-3,4-dihydroxybenzoic acid; 2-NP-2-nitrophenol; 3-NP-3-nitrophenol; 4-NP-4-nitrophenol; the plus/minus values represent standard deviation)
| Enzyme | Catechol 1,2 -dioxygenase, U/mg protein | Catechol 2,3 -dioxygenase, U/mg protein | Protocatechuate 3,4 -dioxygenase U/mg protein | Protocatechuate 4,5 -dioxygenase U/mg protein | |
|---|---|---|---|---|---|
| Inductor | Substrate in reaction mixture | ||||
| Catechol | Catechol | 3,4-DHB | 3,4-DHB | ||
| 2-NP | 3,4-DHB | 0.00 ± 0.00 | 0.06 ± 0.00 | 1.06 ± 0.12 | 0.00 ± 0.00 |
| 4-HB | 0.01 ± 0.00 | 0.00 ± 0.00 | 0.09 ± 0.00 | 0.00 ± 0.00 | |
| Benzoate | 0.01 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | |
| 3-NP | 3,4-DHB | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.26 ± 0.04 | 0.00 ± 0.00 |
| 4-HB | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.81 ± 0.46 | 0.00 ± 0.00 | |
| Benzoate | 0.01 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | |
| 4-NP | 3,4-DHB | 0.00 ± 0.00 | 0.23 ± 0.00 | 0.52 ± 0.12 | 0.00 ± 0.00 |
| 4-HB | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.71 ± 0.65 | 0.00 ± 0.00 | |
| Benzoate | 0.00 ± 0.00 | 0.33 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | |