Figure 3. Comparison of vector distribution resulting from injections of novel adeno-associated virus (AAV) vectors into adult mice.

AAV9, hu.32, hu.37, pi.2, hu.11, hu.48R3, and rh.8 were injected into the cortex, striatum, hippocampus, and thalamus (1 µ l per injection site) of adult mice (>2 months of age), and animals were killed 3 weeks after injection. The highest obtainable vector titers were used for all injections (Table 1). Frozen sections underwent in situ hybridization (ISH) using a riboprobe against the green fluorescent protein sequence. (a) Representative examples of the transduction capabilities of the individual vectors. The injection locations are shown with arrows on the AAV9 sections. The rostral-caudal level of the coronal section relative to bregma is given⁴¹ with (+) indicating that the location is rostral to bregma and a (−) indicating the section is caudal to bregma. Boxes in a, not drawn to scale, correspond to the panels in b. (b) ISH-positive cells can be found in the contralateral hippocampus of AAV9, hu.32, pi.2, hu.11, and rh.8 injected animals, indicating that these vectors were capable of axonal transport from the injected hippocampus.