Table 1.

Selective WGA Enriched for Serratia symbiotica DNA from a Mixed Sample

	Absolute Number of Genic Copies in Sample					DNA Composition from qPCR
	S. symbiotica dnaK	S. symbiotica gyrB	Buchnera dnaK	A. pisum hsp70	A. pisum EF-1α	S. symbiotica Average (%)	Buchnera (%)	A. pisum Average (%)
Tucson genotype aphid DNA	136,800	NA	4,655,000	91,780	NA	0.8	6	93
Filtered Tucson aphid DNA	205,800	NA	2,485,000	1,338	NA	22	53	25
Selectively amplified Tucson DNA	807,500	NA	500,500	445.6	NA	82	10	8
Single read 454 library	788,500	7,499,000	474,000	5,91.9	613.9	95	2	2
Paired read 454 library	618,100	7,847,000	449,200	329.7	806.7	96	2	2
Paired read Illumina library	698,300	5,743,000	787,500	809.3	1,185	91	4	5

Note.—DNA composition was estimated using single gene copy numbers and an estimated genome size of 3 Mb for S. symbiotica. The Buchnera and Acyrthosiphon pisum genomes are 0.6 and 517 Mb in size, respectively (Shigenobu et al. 2000; International Aphid Genomics Consortium 2010).