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. 2001 Jan;12(1):1–12. doi: 10.1091/mbc.12.1.1

Figure 4.

Figure 4

A synthetic peptide derived from the site I sequence of FAK specifically interacts with the SH3 domain of Cas. GST alone (lanes 1 and 2) or fused to the SH3 domain of Cas (lanes 3-8), cortactin (lanes 9 and 10), or Src (lanes 11 and 12), or the N terminus of paxillin (lanes 13 and 14) was preincubated with increasing amounts of wild-type peptide (EAPPKPSRPGYPSPRSS, 0 to 100 μM), followed by incubation with 500 μg of CE cell lysate. Protein complexes were captured, resolved by SDS-PAGE, and blotted for the presence of FAK (top) and FRNK (bottom) with the polyclonal antiserum BC3.