Fig. 2. Purification of TMV MP-interacting protein by affinity chromatography. (A) Protein fractions resolved on a 12.5% SDS–polyacrylamide gel. Protein profile was revealed by silver staining. (B) TMV MP binding on renatured protein blots. Lanes 1, total cell wall extract before loading on the column; lanes 2, flow-through fraction; lanes 3, first 0.5 ml wash fraction; lanes 4, eluted fraction. Arrowhead indicates the position of the 38 kDa TMV MP-interacting protein. The numbers on the left indicate molecular mass standards in kDa.