Fig. 6. Analysis of MP deletion mutants for their ability to bind tobacco PME on renatured blot overlays. (A) Summary of the deletion mutants tested. Ovals indicate retained amino acids; lines indicate deleted amino acids. The numbers in the scale refer to amino acids of the wild-type (wt) TMV MP (Goelet et al., 1982). (B) Renatured blot overlay binding assay using tobacco cell wall fractions. Each lane is designated with the TMV MP derivative whose binding was tested.