Fig. 7. (A) Nucleotide alignment of cDNA clone l3a11 (lower line) and its putative germline gene (cvhhp11, upper line), revealing a deletion of 6 nt (dotted). The palindromic sequence is in bold and underlined. Numbers indicate nucleotide positions of the germline V_HH element. (B) Alignment of the cDNA (lower lines) and the closest germline sequences (upper lines) indicates insertions, which are non-templated (pair 1) or are duplicated (boxed in pair 2). (C) Frequency and distribution of the RSS-like elements in the camel germline V_Hs and V_HHs. Numbers indicate the incidence of RSS found in 50 V_Hs (upper) and 42 V_HHs (lower). (D) Improper joining adjacent to the RSS signal resulting in clones with a deletion: the heptameric sequences are boxed and the expected cleavage site is indicated by an arrow. The three camel V_HH cDNA sequences (l3a08, med64 and hhat20) show a codon deletion when aligned with their putative corresponding genes (cvhhp28 and cvhhp11).