Fig. 3. FADD–DN diminishes CD3ɛ ligation-induced production of CD4⁺8⁺ pre–T cells in rag–1^–/– mice. (A) Flow cytometric analysis of thymocytes from 5- to 13–week-old rag–1^–/–, lpr rag–1^–/– and FADD–DN rag–1^–/– mice after intraperitoneal injection with 100 μg of 145-2C11 hamster anti-mouse CD3ɛ monoclonal antibody. Total thymic cellularity (B) and CD4⁺8⁺ pre–T–cell content (C) were determined by cell counting and flow cytometric analysis of thymocytes stained with antibodies to CD4 and CD8. Each symbol represents one mouse.