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. Author manuscript; available in PMC: 2012 Jan 18.
Published in final edited form as: Dev Cell. 2011 Jan 18;20(1):97–108. doi: 10.1016/j.devcel.2010.11.020

Figure 1.

Figure 1

Rheb1 is Essential for Growth Factor Stimulated mTORC1 Signaling.

(A) Western blots show the failure of serum-starved primary Rheb1−/− MEFs to activate mTORC1 as indicated by pS6K and pS6 in response to insulin stimulation (200nM, 15min).

(B) Western blots reveal normal response of serum-starved Rheb1−/− MEFs to insulin stimulation (200nM, 15min) to activate mTORC2 (pAKT S473) and ERK kinase. IRS1 and 2 are not upregulated in the Rheb1−/− MEFs.

(C) Western blots show the failure of Rheb1−/− primary MEFs to activate mTORC1 activity with AA stimulation (see Supplemental Information).

(D) Immunostaining shows the restoration of the mTORC1 activity in the Rheb1−/− MEFs by myc-Rheb1. DAPI staining (blue) reveals Rheb1−/− MEFs not transfected with Rheb1 transgene.