Rheb1 is not Essential for the Formation of the Brain During Embryonic Development.
(A) Western blots show no changes in neuronal markers (NeuN, Tuj1, Tau1, and MAP2), and astrocyte marker (GFAP) in E17.5 to P5 Rheb1 KO brains. A representative blot from three independent experiments is shown.
(B) Nissl staining reveals the preservation of the gross morphology of the brain structure in the P1 and P5 Rheb1 KO mice, albeit at a reduced size. Bar, 500 μm. Note the reduction in the thickness of the cortex. Bar, 100 μm.
(C) Immunostaining shows normal genesis of oligodendrocyte precursor cells in the corpus callosum of P2 KO brain. Insets show higher magnification of the cells indicated by arrows. Bar, 20 μm. Data represent mean ± SEM. Results are averages of two independent experiments.