Differentiation of OPCs into Postmitotic Oligodendrocytes is Impaired in the Rheb1 KO.
(A) and (B) Immunostaining shows the reduction in the number of postmitotic oligodendrocytes (CC1+Olig2+) in the corpus callosum of 2~3-wk old Rheb1 KO brain (n=3). Bar, 20 μm. Data represent mean ± SEM. **p<0.01.
(C) and (D) Immunostaining shows increased number of OPCs in the cortex, but normal number of OPCs in the corpus callosum of 3-wk old Rheb1 KO brain (n=3). Arrows indicate the NG2/PDGFRα double positive cells. Bar, 20 μm. Data represent mean ± SEM. **p<0.01.
(E) Western blots show increasing amount of NG2 proteins in the cortex, hippocampus, and cerebellum of 3~4-wk old Rheb1 KO mice.