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. Author manuscript; available in PMC: 2012 Jan 18.
Published in final edited form as: Dev Cell. 2011 Jan 18;20(1):97–108. doi: 10.1016/j.devcel.2010.11.020

Figure 6.

Figure 6

Rheb1 Transgene Promotes Myelination.

(A) Western blots show increasing amount of myelin proteins in the brain of 2-wk old Rheb1 transgenic (Tg, k/k, Nes-cre) mice relative to controls (k/k or k/+) and comparable amount of myelin proteins in the 3-month old Rheb1 Tg mice.

(B) and (C) Histograms show quantifications of myelin protein and mTORC1 activity in the cortex of Rheb1 Tg mice. Data represent mean ± SEM. Results are averages of three independent experiments.

(D) Immunostaining with MBP antibody shows enhanced myelination in the corpus callosum (CC), cingulum bundles (Cing) and cortex (CTX) at P7 and P14 and normal myelination at P28. Arrows indicate the MBP positive labeling. Bar, 20 μm.

(E) and (F) Immunostaining with CC1 and Olig2 antibody shows increased postmitotic oligodendrocytes in the 1-wk old Rheb1 Tg mice compared with controls (k/k; k/+). Arrows indicate the NG2/PDGFRα double positive cells. Bar, 50 μm. Data represent mean ± SEM. Results are averages of three independent experiments.

(G) Histogram shows comparable number of postmitotic oligodendrocytes in the 2-wk old Rheb1 Tg mice revealed by immunostaing. Data represent mean ± SEM. Results are averages of three independent experiments.