Fig. 4.
Assessment of NR2A and NR2B expression in the PrV by Western blot analysis. (A) NR2A and NR2B protein levels at P5 and P14 in the developing and denervated PrV. (B) NR2A and NR2B expression on the normal side of the PrV at different ages. The intensity measurements of NR2A and NR2B were normalized to actin levels and expressed as relative intensity. The averaged relative intensity is expressed by mean±S.E.M. In the normal PrV (control), NR2A and NR2B expressions are about the same at P5 and P14 (ANOVA test p=0.97); suggesting that there is no developmental change in NR2A and NR2B levels and no significant difference (n=5, p=0.97) between NR2A and NR2B expression. (C) Comparison of NR2A expression on the normal and denervated sides at different ages. ANOVA test (p=0.75) shows no significant difference between both sides. (D) Comparison of NR2B expression on the normal and denervated sides of the PrV at different ages. ANOVA test (p=0.97) shows no significant difference between both sides.