Intrapulse data. (A) Peak particle velocity within a pulse is reported here as a mean of medians in dB SPVL, using 50 nm s−1 as a reference. There no significant differences were detected in dB SPVL, but there was a trend for a reduction in pulse amplitude for tshGAL4/UAS-fruMIR males. (B) Peak frequency of sine songs (circle) and individual pulses (triangle). No significant differences were observed. (C) Pulse widths from tshGAL4/UAS-fruMIR males were significantly broader compared to tshGAL4/+ controls. This broadened pulse width is rescued in tshGAL4/UAS-fruMIR; n-sybGAL80 males. (D) Mean interpusle interval (IPI) was unaffected by genotype. *: p < 0.05, **: p < 0.01, ‡: one-tailed, p < 0.05.