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. 2001 Jan;12(1):13–26. doi: 10.1091/mbc.12.1.13

Figure 2.

Figure 2

CPY sorting and Vps10p stability are defective in ric1Δ and ypt6Δ strains. (A) CPY sorting. Wild-type (WT, SEY6210), ric1Δ (GPY1701) and ypt6Δ (GPY1700) strains were grown and subjected to pulse-chase analysis as described in the legend to Figure 1B. After a 15-min labeling period, samples were removed after 0, 10, and 30 min of chase and CPY was immunoprecipitated from internal (I) and external (E) fractions. CPY was resolved by SDS-PAGE and subjected to phophorimage analysis. (B) Vps10p stability. Wild-type (WT, SEY6210), ric1Δ (GPY1480), and ric1Δ pep4Δ (GPY1492) strains were grown and subjected to pulse-chase analysis as described in the legend to Figure 1B. Following a 10-min labeling period, cells were removed after 0, 60, and 120 min of chase and Vps10p was immunoprecipitated from cell lysates. Vps10p was visualized by SDS-PAGE followed by autoradiography. The 170-kDa Vps10p proteolytic cleavage product is denoted by an asterisk.