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. 2001 Jan;12(1):13–26. doi: 10.1091/mbc.12.1.13

Figure 3.

Figure 3

Vps10p is mislocalized in ric1Δ cells. Wild-type (WT, SEY6210) and ric1Δ (GPY1408) cells were grown at 30°C, converted to spheroplasts, and subjected to differential centrifugation. S2 and P2 are, respectively, the supernatant and pellet fractions from centrifugation at 10,000 × g for 15 min.; S3 and P3 are, respectively, the supernatant and pellet fractions from centrifugation at 200,00 × g for 17 min. Pellets were resuspended in volumes equal to the supernatants, and equal volumes were analyzed by SDS-PAGE and immunoblotting.