Figure 4.

Efficient transport of ALP to the vacuole in ric1Δ and ric1Δ end3Δ cells. Wild-type (WT, SEY6210), ric1Δ (GPY1480), and ric1Δ end3Δ (GPY1609) strains were grown and subjected to pulse-chase analysis as described in the legend to Figure 1B. After a 10-min labeling period, cells were removed at 0, 15, and 30 min after the addition of chase, and ALP was immunoprecipitated from cell lysates. ALP was visualized by SDS-PAGE followed by autoradiography. P and M, precursor and mature forms of ALP, respectively.