Fig. 7. Homomeric complex formation of Xdsh: membrane co-translocation of translocation-defective deletions with wild-type Xdsh. mRNAs for Xdsh-HA (A and D) and D6–GFP (B and E) were co-injected in early stage embryos without (A–C) or with rat frizzled-1 mRNA (D–F). The subcellular location of mutant and wild-type Xdsh in gastrula animal caps is shown. Images on the left (A and D) show the fluorescence of rhodamine-labeled Xdsh-HA (red channel), middle images (B and E) show the GFP fluorescence of D6–GFP (green channel) in the same animal cap tissue; and images on the right (C and F) are overlays of both signals. Note that D6–GFP (E) is found associated with the membrane when it is co-injected with Xdsh-HA and frizzled, while it remains cytoplasmic/vesicular when co-injected with frizzled alone (compare Figure 4J). D8-HA (G and J) co-injection with Xdsh–GFP (H and K) results in D8-HA accumulation at the membrane, when co-injected with frizzled (J–L). D8 on its own with frizzled does not localize well to the membrane (data not shown). Left panels (red channel) are Texas red signals of D8-HA (G and J); middle panels (green channel) are GFP signals (H and K) and right panels are overlays of both signals (I and L).