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. Author manuscript; available in PMC: 2011 Mar 14.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Jul 17;399(2):117–122. doi: 10.1016/j.bbrc.2010.07.009

Fig. 1.

Fig. 1

A base substitution in pri-miR-126 in RS4;11 cells is identified. (A) DNA chromatograms illustrating the genomic sequence of miR-126 DNA from RS4;11 cells. Nucleotides are indicated by capital letters. The green, black, and blue wave indicates A, G, and C, respectively. The arrows indicate the base position of A24G base substitution. Note that G and A sequence are both present. The base substitution is located 24 bp from the 3′end of mature miR-126 miRNA. (B) The location of the base substitution in the pri-miR-126. The red capital indicates A24G. (C) The structure of the pXZ201-pri-miR-126. Pri-miR-126 genomic sequence was cloned into the pXZ201 retroviral expression vector. (D) The structure of the pri-miR-126 cloned from the genome of RS4;11 cells. 5′ and 3′ flanking regions was 134 and 117 base long, respectively, which are enough length for processed from pri-miRNA to pre-miRNA [27].