Fig 2.

Glioma-associated antigen (GAA) –specific T-cell responses evaluated by interferon (IFN) -γ enzyme-linked immunosorbent spot (ELISPOT) and tetramer analyses. (A) Time course for IFN-γ ELISPOT assays for all evaluated patients with box plots (boxes = 25th to 75th percentiles; vertical lines = minimum to maximum). Numbers at the bottom of each time point in the panel for YKL-40 are the number of assessable patients at the time shown. These numbers also pertain to the other GAAs and a pan-DR epitope (PADRE). (B) IFN-γ ELISPOT analysis for patient 10. (C) Patient 6 showed durable tetramer responses, which were analyzed for up to 33 weeks. Examples of histograms for positive tetramer responses against the interleukin (IL) -13Rα2-epitope are shown.