Figure 4.
Hfe is expressed in erythroid progenitors. (A) Fluorescence-activated cell sorter profiles of wt splenic erythroid cells after selection. (Top panel) Tfrc and Ter119 costaining. (Bottom panel) CD44 versus forward scatter (FSC) as previously described by Chen et al.31 (B) Reverse-transcribed polymerase chain reaction showing expression of Hfe, Tfr2, Fpn1, and β-actin in erythroid cells harvested from the spleens of phlebotomized wt mice and (C) in erythroid colonies derived from BM cells.