Figure 1.

NMuMG cells treated with TGF-β undergo EMT. (A) Immunoblotting of NMuMG cells incubated with 5 ng/ml TGF-β for 0–48 h shows nearly equivalent cellular expression of E-cadherin and β-catenin through the time course, whereas N-cadherin expression is induced by 3 h of TGF-β treatment and remains elevated throughout the time course. (B) Epifluorescent and corresponding phase contrast images show that the fibroblastic cell morphology of cells treated with 5 ng/ml TGF-β for 24 h have a loss of immunodetectable E-cadherin at the cell junctions with a gain of N-cadherin expression at cell margins.